1-Site Ligand Binding Curve

This tutorial will show how to use the TechGraph Editor to fit a 1-Site Ligand Binding curve to a set of XY data points.  Shown below are the settings in the Data Table and the Edit Chart used to create this chart.        

Data Table – Sample data can easily be changed or replaced by pasting data from an external worksheet.

• Graph Type is Scatter.
• X Data Type is Number.  X Data column contains the X values.
• Graph A series is set to Scatter and it contains the Y values.

Note:   Click on the “Help” button on the toolbar to find out how to edit, delete, copy and paste in the Data Table.

Edit Chart – A set of icons (Titles, Settings, X Axis, Y Axis, Curves and Error Bars) to change the settings and appearance of the chart.      

     Settings  – specify the color, marker type and size for Graph A marker.         

•  Click on the “DarkOrange circle” (Graph Style) to show the marker Style for Graph A pop-up.   You can change the Color, Type and Size for Graph A marker.   Click on Apply to save the changes.

     X Axis 

• Enter values in Minimum, Maximum and Major Div to set the X Axis scale and divisions.
  • Values for this example are Minimum = 0, Maximum = 150 and  Major Div = 5.

     Y axis

• Enter values in Minimum, Maximum and Major Div to set the Y Axis scale and divisions. Leave these values empty for automatic scaling.

     Curves & Error Bars       

•  Click on “1-Site Ligand” (Curve Fit) for Graph A to show the Curvefit Selection for Graph A pop-up.  For this example, “1-Site Ligand” is selected from the Selection list. Click on Apply to save the changes.
• Click on Curve Style for Graph A to show the Curve Style for Graph A pop-up.  Curve Color (“black”) is selected for the 1-Site Ligand curve color.  Use the Thickness and Line Type selection list to change the line style. Click on Apply to save the changes

    Edit Chart Toolbar Options

•  Print Chart prints your chart.
•  Download Image downloads your chart as an SVG(Scalable Vector Graphics) file or image file(PNG, JPEG, TIF, PDF).
•  Drawing Objects customize your chart with labels, lines, arrows, circles, images, and logos.

 Getting Started:

• Data Table, Fill Data, Data Transform, Linear Curve Fitter, Nonlinear Curve Fitter
• Edit Chart, Drawing Objects, Chart Setup
• Titles, Settings, X Axis, Y Axis, Curves and Error Bars

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